Improvement and application of a polymerase chain reaction system for detection of Wuchereria bancrofti in Culex quinquefasciatus and human blood samples.
نویسندگان
چکیده
منابع مشابه
A polymerase chain reaction assay for the survey of bancroftian filariasis.
A polymerase chain reaction (PCR) assay based on a highly repeated DNA sequence found in Wuchereria bancrofti (SspI repeat) has been modified for the survey of bancroftian filariasis in expatriate workers (Myamese, Karen and Mon) from Myanmar where human filariasis is endemic. The PCR was very sensitive with the ability to detect the presence of as little as 10 pg of parasite DNA. The primers u...
متن کاملExperimental infection of Culex (Culex) quinquefasciatus and Aedes (Stegomyia) aegypti with Wuchereria bancrofti.
A study was conducted to determine the susceptibility of local strains of Culex quinquefasciatus and Aedes aegypti to infection with the strain of Wuchereria bancrofti that occurs in Maceió. State of Alagoas, Brazil. Cx. quinquefasciatus blood fed simultaneously on the same microfilariae carrier ingested more blood and 2-3x more microfilariae than Ae. aegypti. Survival rates of both species of ...
متن کاملAssessment of family and neighbors of an individual infected with Wuchereria bancrofti from a non-endemic area in the city of Maceió, Brazil.
The family and neighbors of a patient infected with W. bancrofti microfilariae were assessed aiming to evaluate the occurrence of cases of lymphatic filariasis in a non-endemic area in the city of Maceió, in the Brazilian state of Alagoas. The patient had previously lived in an endemic focus; however, he has been living in an area where the parasite has never been detected for the past ten year...
متن کاملCulex quinquefasciatus in Phitsanulok as a possible vector of nocturnally periodic Wuchereria bancrofti transmission in Myanmar immigrants.
The present study was undertaken in order to study whether Culex quinquefasciatus collected in Phitsanulok Province can be an insect host for the development of Wuchereria bancrofti larvae. W. bancrofti infected blood from Myanmar workers in Mae Sot, Tak Province was fed to mosquitoes by using the artificial membrane feeding. An infection of W. bancrofti was found with the highest density of L3...
متن کاملA real-time PCR-based assay for detection of Wuchereria bancrofti DNA in blood and mosquitoes.
We developed and evaluated real-time polymerase chain reaction (PCR) assays for detecting Wuchereria bancrofti DNA in human blood and in mosquitoes. An assay based on detection of the W. bancrofti "LDR" repeat DNA sequence was more sensitive than an assay for Wolbachia 16S rDNA. The LDR-based assay was sensitive for detecting microfilarial DNA on dried membrane filters or on filter paper. We al...
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ورودعنوان ژورنال:
- Memorias do Instituto Oswaldo Cruz
دوره 92 1 شماره
صفحات -
تاریخ انتشار 1997